IkappaB kinase-beta: NF-kappaB activation and complex formation with IkappaB kinase-alpha and NIK.
Activation of the transcription factor nuclear factor kappa B (NF-kappaB) by inflammatory cytokines requires the successive action of NF-kappaB-inducing kinase (NIK) and IkappaB kinase-alpha (IKK-alpha). A widely expressed protein kinase was identified that is 52 percent identical to IKK-alpha. IkappaB kinase-beta (IKK-beta) activated NF-kappaB when overexpressed and phosphorylated serine residues ... 32 and 36 of IkappaB-alpha and serines 19 and 23 of IkappaB-beta. The activity of IKK-beta was stimulated by tumor necrosis factor and interleukin-1 treatment. IKK-alpha and IKK-beta formed heterodimers that interacted with NIK. Overexpression of a catalytically inactive form of IKK-beta blocked cytokine-induced NF-kappaB activation. Thus, an active IkappaB kinase complex may require three distinct protein kinases.
Mesh Terms:
Amino Acid Sequence, Cell Line, Cytokines, Enzyme Activation, Genes, Reporter, Hela Cells, Humans, I-kappa B Kinase, Molecular Sequence Data, NF-kappa B, Phosphorylation, Protein-Serine-Threonine Kinases, Sequence Homology, Amino Acid, Transfection
Amino Acid Sequence, Cell Line, Cytokines, Enzyme Activation, Genes, Reporter, Hela Cells, Humans, I-kappa B Kinase, Molecular Sequence Data, NF-kappa B, Phosphorylation, Protein-Serine-Threonine Kinases, Sequence Homology, Amino Acid, Transfection
Science
Date: Oct. 31, 1997
PubMed ID: 9346485
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