A molecular dissection of the repression circuitry of Ikaros.
Ikaros is a key regulator of the hemo-lymphoid system in which it is presumed to function by both potentiating and repressing gene expression. Repression is mediated through two independent domains at the N and C terminus of the protein, both of which can independently recruit the corepressors Mi-2beta, Sin3A, and ... Sin3B and the Class I histone deacetylases 1 and 2; the N-terminal domain can also associate with the corepressor CtBP. Here we describe a detailed dissection of these two domains and identify the minimal repression modules and the corepressor requirements for their activity. Based on these studies, we describe mutations in a full-length Ikaros protein that abrogate interactions with each of the identified corepressors and abolish the protein's function as a repressor. Finally, we show that, barring CtBP, the Ikaros family members Aiolos, Helios, and Eos can associate with all of the identified corepressors of Ikaros including its newly identified interactors, Class II HDACs.
Mesh Terms:
3T3 Cells, Animals, Cell Line, Cloning, Molecular, DNA-Binding Proteins, Humans, Ikaros Transcription Factor, Mice, Mutagenesis, Protein Isoforms, Recombinant Fusion Proteins, Recombinant Proteins, Repressor Proteins, Sequence Deletion, Transcription Factors, Transfection, Zinc Fingers
3T3 Cells, Animals, Cell Line, Cloning, Molecular, DNA-Binding Proteins, Humans, Ikaros Transcription Factor, Mice, Mutagenesis, Protein Isoforms, Recombinant Fusion Proteins, Recombinant Proteins, Repressor Proteins, Sequence Deletion, Transcription Factors, Transfection, Zinc Fingers
J. Biol. Chem.
Date: Aug. 02, 2002
PubMed ID: 12015313
View in: Pubmed Google Scholar
Download Curated Data For This Publication
11671
Switch View:
- Interactions 32