p300/CREB-binding protein interacts with ATR and is required for the DNA replication checkpoint.
The highly related acetyltransferases, p300 and CREB-binding protein (CBP) are coactivators of signal-responsive transcriptional activation. In addition, recent evidence suggests that p300/CBP also interacts directly with complexes that mediate DNA replication and repair. In this report, we show that loss of p300/CBP in mammalian cells results in a defect in ... the cell cycle arrest induced by stalled DNA replication. We demonstrate that complexes containing p300/CBP and ATR can be detected in mammalian cells, and that the downstream kinase CHK1 fails to be phosphorylated in response to stalled DNA replication in cells that lack p300/CBP. These observations broaden the roles for the p300/CBP acetyltransferases to include the modulation of chromatin structure and function during DNA metabolic events as well as for transcription.
Mesh Terms:
CREB-Binding Protein, Cell Cycle Proteins, DNA Replication, Hela Cells, Histone Acetyltransferases, Humans, Protein-Serine-Threonine Kinases, Transcription Factors, p300-CBP Transcription Factors
CREB-Binding Protein, Cell Cycle Proteins, DNA Replication, Hela Cells, Histone Acetyltransferases, Humans, Protein-Serine-Threonine Kinases, Transcription Factors, p300-CBP Transcription Factors
J. Biol. Chem.
Date: Mar. 30, 2007
PubMed ID: 17272271
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