Identification of an IQGAP1/AKAP79 complex in beta-cells.

IQGAP1, is a recently discovered scaffold protein proposed to regulate membrane cytoskeleton events through protein-protein interactions with F-actin, E-cadherin, beta-catenin, and CLIP170. The binding of IQGAP1 to its partners is regulated by calcium/calmodulin (Ca(++)/CaM) and the small molecular weight guanine nucleotide triphosphatases (GTPases), Cdc42, and Rac1. Here we identify a ...
novel IQGAP1 scaffolding function by isolating the cyclic AMP dependent kinase (PKA) with IQGAP1. IQGAP1 was co-purified with PKA using 5'-cyclic AMP (cAMP) affinity chromatography and PKA activity was co-immunoprecipitated with IQGAP1 using an anti-IQGAP1 antibody. The association of IQGAP1 with PKA was shown to occur through a direct interaction between A kinase anchoring protein 79 (AKAP79) and the carboxyl-terminal domain of IQGAP1. This suggests that cAMP/PKA may be coupled with Ca(++)/CaM and GTPases through an IQGAP1/AKAP79 complex.
Mesh Terms:
A Kinase Anchor Proteins, Adaptor Proteins, Signal Transducing, Animals, Calcium, Calmodulin, Carrier Proteins, Cattle, Cell Membrane, Cells, Cultured, Cyclic AMP, Cyclic AMP-Dependent Protein Kinases, Humans, Islets of Langerhans, Macromolecular Substances, Protein Binding, Protein Structure, Tertiary, Rats, Recombinant Proteins, Signal Transduction, cdc42 GTP-Binding Protein, rac GTP-Binding Proteins, ras GTPase-Activating Proteins
J. Cell. Biochem.
Date: Sep. 01, 2003
Download Curated Data For This Publication
11718
Switch View:
  • Interactions 3