MALS is a binding partner of IRSp53 at cell-cell contacts.
Insulin receptor substrate p53 (IRSp53) is a key player in cytoskeletal dynamics, interacting with the actin modulators WAVE2 and Mena. Here, we identified a PDZ protein, MALS, as an IRSp53-interacting protein using a yeast two-hybrid screen. A pull-down assay showed that IRSp53 and MALS interact through the PDZ domain of ... MALS and the C-terminal PDZ-binding sequence of IRSp53. Their interaction in MDCK cells was also demonstrated by co-immunoprecipitation. Immunocytochemistry showed the colocalization of IRSp53 and MALS at cell-cell contacts. Cytochalasin D induced the redistribution of both proteins to the cytosol. Thus, MALS is a partner of IRSp53 anchoring the actin-based membrane cytoskeleton at cell-cell contacts.
Mesh Terms:
Amino Acid Sequence, Animals, Binding Sites, Carrier Proteins, Cell Communication, Cytochalasin D, Cytoskeletal Proteins, Cytoskeleton, Immunohistochemistry, Membrane Proteins, Nerve Tissue Proteins, Protein Binding, Rats, Two-Hybrid System Techniques
Amino Acid Sequence, Animals, Binding Sites, Carrier Proteins, Cell Communication, Cytochalasin D, Cytoskeletal Proteins, Cytoskeleton, Immunohistochemistry, Membrane Proteins, Nerve Tissue Proteins, Protein Binding, Rats, Two-Hybrid System Techniques
FEBS Lett.
Date: Nov. 06, 2003
PubMed ID: 14596909
View in: Pubmed Google Scholar
Download Curated Data For This Publication
11723
Switch View:
- Interactions 8