Definition of the consensus motif recognized by gamma-adaptin ear domains.

The heterotetrameric adaptor complex 1 (AP-1) and the monomeric Golgi-localized, gamma ear-containing, Arf-binding (GGA) proteins are components of clathrin coats associated with the trans-Golgi network and endosomes. The carboxyl-terminal ear domains (or gamma-adaptin ear (GAE) domains) of two gamma-adaptin subunit isoforms of AP-1 and of the GGAs are structurally similar ...
and bind to a common set of accessory proteins. In this study, we have systematically defined a core tetrapeptide motif PsiG(P/D/E)(Psi/L/M) (where Psi is an aromatic residue), which is responsible for the interactions of accessory proteins with GAE domains. The definition of this motif has allowed us to identify novel GAE-binding partners named NECAP and aftiphilin, which also contain clathrin-binding motifs. These findings shed light on the mechanism of accessory protein recruitment to trans-Golgi network and endosomal clathrin coats.
Mesh Terms:
Adaptor Protein Complex 1, Adaptor Protein Complex gamma Subunits, Adaptor Proteins, Vesicular Transport, Amino Acid Sequence, Animals, Binding Sites, Biotinylation, Carrier Proteins, Clathrin, Consensus Sequence, Glutathione Transferase, Green Fluorescent Proteins, Humans, Luminescent Proteins, Mice, Microscopy, Fluorescence, Molecular Sequence Data, Mutagenesis, Peptide Fragments, Peptide Library, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Structure-Activity Relationship, Two-Hybrid System Techniques
J. Biol. Chem.
Date: Feb. 27, 2004
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