Association of SLP-65/BLNK with the B cell antigen receptor through a non-ITAM tyrosine of Ig-alpha.
The cytoplasmic adaptor protein SLP-65 (BLNK or BASH) is a critical downstream effector of the B cell antigen receptor (BCR). Tyrosine-phosphorylated SLP-65 assembles intracellular signaling complexes such as the Ca(2 +) initiation complex encompassing phospholipase C-gamma2 and Bruton's tyrosine kinase. It is, however, unclear how the SLP-65 signaling module can ... be recruited to the plasma membrane. Here we show that following B cell stimulation, SLP-65 associates directly with the BCR signaling subunit, the Ig-alpha / Ig-beta heterodimer. The interaction is mediated by the Src homology 2 domain of SLP-65 and the phosphorylated Ig-alpha tyrosine 204, which is located outside of the immunoreceptor tyrosine-based activation motif. Our data identify an unexpected BCR phosphorylation pattern and indicate that Ig-alpha has the capability to serve as transmembrane adaptor in BCR signaling.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Amino Acid Motifs, Amino Acid Sequence, Animals, Antigens, CD, Antigens, CD79, B-Lymphocytes, Carrier Proteins, Cell Line, Models, Immunological, Molecular Sequence Data, Phosphoproteins, Phosphorylation, Phosphotyrosine, Receptors, Antigen, B-Cell, Sequence Alignment, src Homology Domains
Adaptor Proteins, Signal Transducing, Amino Acid Motifs, Amino Acid Sequence, Animals, Antigens, CD, Antigens, CD79, B-Lymphocytes, Carrier Proteins, Cell Line, Models, Immunological, Molecular Sequence Data, Phosphoproteins, Phosphorylation, Phosphotyrosine, Receptors, Antigen, B-Cell, Sequence Alignment, src Homology Domains
Eur. J. Immunol.
Date: Jul. 01, 2001
PubMed ID: 11449366
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