Cohesion establishment factor, Eco1 represses transcription via association with histone demethylase, LSD1.
Accurate chromosome segregation during cell division requires physical attachment of two sister chromatids from DNA replication until mitosis, a process known as sister chromatid cohesion. Sister chromatid cohesion is mediated by a four-subunit cohesin complex that connects sister chromatids by encircling them as molecular rings. Eco1 is an essential acetyltransferase ... required for the establishment of sister chromatid cohesion. Eco1 acetylates Smc3 and Mcd1 (Rad21) to establish cohesion during S phase and in response to DNA damage, respectively. Here, we show that Eco1 repress transcription in a dose-dependent manner. Mutations of zinc-finger domain or acetyltransferase domain did not significantly affect the transcriptional repression activity of Eco1. We observed that histone demethylase, Lsd1 interacts with Eco1 and is required for the transcriptional repression activity of Eco1. Chromatin immunoprecipitation (ChIP) experiments show that Eco1 facilitates the demethylation of lysine 4 of histone H3. Taken together, we provide the first evidence that Eco1 represses transcription by interacting with histone demethylase, LSD1 to convert chromatin to inactive state.
Mesh Terms:
Acetyltransferases, Cell Line, Chromatin, Chromatin Immunoprecipitation, Gene Expression Regulation, Histone Deacetylase 1, Histone Deacetylase 2, Histone Demethylases, Humans, Lysine, Methylation, Repressor Proteins, Transcription, Genetic
Acetyltransferases, Cell Line, Chromatin, Chromatin Immunoprecipitation, Gene Expression Regulation, Histone Deacetylase 1, Histone Deacetylase 2, Histone Demethylases, Humans, Lysine, Methylation, Repressor Proteins, Transcription, Genetic
Biochem. Biophys. Res. Commun.
Date: Apr. 16, 2010
PubMed ID: 20331966
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