Kv2.1/Kv9.3, a novel ATP-dependent delayed-rectifier K+ channel in oxygen-sensitive pulmonary artery myocytes.
The molecular structure of oxygen-sensitive delayed-rectifier K+ channels which are involved in hypoxic pulmonary artery (PA) vasoconstriction has yet to be elucidated. To address this problem, we identified the Shab K+ channel Kv2.1 and a novel Shab-like subunit Kv9.3, in rat PA myocytes. Kv9.3 encodes an electrically silent subunit which ... associates with Kv2.1 and modulates its biophysical properties. The Kv2.1/9.3 heteromultimer, unlike Kv2.1, opens in the voltage range of the resting membrane potential of PA myocytes. Moreover, we demonstrate that the activity of Kv2.1/Kv9.3 is tightly controlled by internal ATP and is reversibly inhibited by hypoxia. In conclusion, we propose that metabolic regulation of the Kv2.1/Kv9.3 heteromultimer may play an important role in hypoxic PA vasoconstriction and in the possible development of PA hypertension.
Mesh Terms:
Adenosine Triphosphate, Amino Acid Sequence, Anaerobiosis, Animals, Cloning, Molecular, Delayed Rectifier Potassium Channels, Ion Channel Gating, Molecular Sequence Data, Muscle, Smooth, Vascular, Oxygen, Phylogeny, Potassium Channels, Potassium Channels, Voltage-Gated, Protein Binding, Pulmonary Artery, Rats, Rats, Sprague-Dawley, Recombinant Proteins, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Shab Potassium Channels
Adenosine Triphosphate, Amino Acid Sequence, Anaerobiosis, Animals, Cloning, Molecular, Delayed Rectifier Potassium Channels, Ion Channel Gating, Molecular Sequence Data, Muscle, Smooth, Vascular, Oxygen, Phylogeny, Potassium Channels, Potassium Channels, Voltage-Gated, Protein Binding, Pulmonary Artery, Rats, Rats, Sprague-Dawley, Recombinant Proteins, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Shab Potassium Channels
EMBO J.
Date: Nov. 17, 1997
PubMed ID: 9362476
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