Staring, a novel E3 ubiquitin-protein ligase that targets syntaxin 1 for degradation.
Syntaxin 1 is an essential component of the neurotransmitter release machinery, and regulation of syntaxin 1 expression levels is thought to contribute to the mechanism underlying learning and memory. However, the molecular events that control the degradation of syntaxin 1 remain undefined. Here we report the identification and characterization of ... a novel RING finger protein, Staring, that interacts with syntaxin 1. Staring is expressed throughout the brain, where it exists in both cytosolic and membrane-associated pools. Staring binds and recruits the brain-enriched E2 ubiquitin-conjugating enzyme UbcH8 to syntaxin 1 and facilitates the ubiquitination and proteasome-dependent degradation of syntaxin 1. These findings suggest that Staring is a novel E3 ubiquitin-protein ligase that targets syntaxin 1 for degradation by the ubiquitin-proteasome pathway.
Mesh Terms:
Amino Acid Sequence, Animals, Antigens, Surface, Blotting, Northern, Blotting, Western, Cloning, Molecular, DNA, Complementary, Humans, Hydrolysis, Ligases, Molecular Sequence Data, Nerve Tissue Proteins, Rats, Sequence Homology, Amino Acid, Syntaxin 1, Ubiquitin-Protein Ligases
Amino Acid Sequence, Animals, Antigens, Surface, Blotting, Northern, Blotting, Western, Cloning, Molecular, DNA, Complementary, Humans, Hydrolysis, Ligases, Molecular Sequence Data, Nerve Tissue Proteins, Rats, Sequence Homology, Amino Acid, Syntaxin 1, Ubiquitin-Protein Ligases
J. Biol. Chem.
Date: Sep. 20, 2002
PubMed ID: 12121982
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