Functional analysis of transcriptional repressor Otx3/Dmbx1.
Otx3/Dmbx1 is a member of paired class homeodomain transcription factors. In this study, we found that Otx3/Dmbx1 represses the Otx2-mediated transactivation by forming heterodimer with Otx2 on the P3C (TAATCCGATTA) sequence in vitro. The 156 amino acid region (residues 1-156) of Otx3/Dmbx1 is required for its repressor activity, and interacts ... directly with Otx2. Co-localization of Otx3/Dmbx1 and Otx2 in brain sections was confirmed by in situ hybridization. These data suggest that Otx3/Dmbx1 represses Otx2-mediated transcription in the developing brain. We also identified the consensus binding sequence [TAATCCGATTA and TAATCC(N2-4)TAATCC] of Otx3/Dmbx1.
Mesh Terms:
Animals, Base Sequence, DNA Primers, Dimerization, Homeodomain Proteins, Mice, Nerve Tissue Proteins, Otx Transcription Factors, Repressor Proteins, Transcription, Genetic
Animals, Base Sequence, DNA Primers, Dimerization, Homeodomain Proteins, Mice, Nerve Tissue Proteins, Otx Transcription Factors, Repressor Proteins, Transcription, Genetic
FEBS Lett.
Date: May. 23, 2005
PubMed ID: 15890343
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