Hrs-2 regulates receptor-mediated endocytosis via interactions with Eps15.
Hrs-2, via interactions with SNAP-25, plays a regulatory role on the exocytic machinery. We now show that Hrs-2 physically interacts with Eps15, a protein required for receptor-mediated endocytosis. The Hrs-2/Eps15 interaction is calcium dependent, inhibited by SNAP-25 and alpha-adaptin, and results in the inhibition of receptor-mediated endocytosis. Immunoelectron microscopy reveals ... Hrs-2 localization on the limiting membrane of multivesicular bodies, organelles in the endosomal pathway. These data show that Hrs-2 regulates endocytosis, delineate a biochemical pathway (Hrs-2-Eps15-AP2) in which Hrs-2 functions, and suggest that Hrs-2 acts to provide communication between endo- and exocytic processes.
Mesh Terms:
Adenosine Triphosphatases, Animals, Brain, Calcium, Calcium-Binding Proteins, Cerebellum, Endocytosis, Endosomal Sorting Complexes Required for Transport, Endosomes, Hela Cells, Humans, Intracellular Signaling Peptides and Proteins, Kinetics, Membrane Proteins, Microscopy, Immunoelectron, Nerve Tissue Proteins, Neurons, Phosphoproteins, Rats, Receptors, Cell Surface, Recombinant Proteins, Signal Transduction, Synaptosomal-Associated Protein 25
Adenosine Triphosphatases, Animals, Brain, Calcium, Calcium-Binding Proteins, Cerebellum, Endocytosis, Endosomal Sorting Complexes Required for Transport, Endosomes, Hela Cells, Humans, Intracellular Signaling Peptides and Proteins, Kinetics, Membrane Proteins, Microscopy, Immunoelectron, Nerve Tissue Proteins, Neurons, Phosphoproteins, Rats, Receptors, Cell Surface, Recombinant Proteins, Signal Transduction, Synaptosomal-Associated Protein 25
J. Biol. Chem.
Date: May. 19, 2000
PubMed ID: 10809762
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