Leukemia-associated Rho guanine nucleotide exchange factor (LARG) links heterotrimeric G proteins of the G(12) family to Rho.
A putative guanine nucleotide exchange factor (GEF), termed leukemia-associated RhoGEF (LARG), was recently identified upon fusion to the coding sequence of the MLL gene in acute myeloid leukemia. Although the function of LARG is still unknown, it exhibits a number of structural domains suggestive of a role in signal transduction, ... including a PDZ domain, a LH/RGS domain, and a Dbl homology/pleckstrin homology domain. Here, we show that LARG can activate Rho in vivo. Furthermore, we present evidence that LARG is an integral component of a novel biochemical route whereby G protein-coupled receptors (GPCRs) and heterotrimeric G proteins of the G alpha(12) family stimulate Rho-dependent signaling pathways.
Mesh Terms:
Cloning, Molecular, Guanine Nucleotide Exchange Factors, Guanosine Diphosphate, Guanosine Triphosphate, Heterotrimeric GTP-Binding Proteins, Humans, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction, Transfection, rho GTP-Binding Proteins
Cloning, Molecular, Guanine Nucleotide Exchange Factors, Guanosine Diphosphate, Guanosine Triphosphate, Heterotrimeric GTP-Binding Proteins, Humans, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction, Transfection, rho GTP-Binding Proteins
FEBS Lett.
Date: Nov. 24, 2000
PubMed ID: 11094164
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