The mouse FKBP23 binds to BiP in ER and the binding of C-terminal domain is interrelated with Ca2+ concentration.

FK506 binding protein 23 from mouse (mFKBP23) is a peptidyl-prolyl cis-trans isomerase (PPIase) from the endoplasmic reticulum (ER), which consists of an N-terminal PPIase domain and a C-terminal domain with Ca(2+) binding sites. The assay of adsorption from ER extract with glutathione S-transferase-mFKBP23 attached to glutathione-Sepharose 4B shows that mFKBP23 ...
binds to mouse immunoglobulin binding protein (mBiP). The same assay with the recombinant proteins of the N- and C-termini of mFKBP23 shows that the binding of the C-terminus is Ca(2+)-dependent and the switch point is between 2 and 3 mM. By high concentration of Ca(2+) this binding cannot be detected. Furthermore, the Ca(2+)-regulated binding of mFKBP23 and mBiP in ER can be detected by means of co-immunoprecipitation.
Mesh Terms:
Animals, Calcium, Calcium-Binding Proteins, Carrier Proteins, Endoplasmic Reticulum, HSP70 Heat-Shock Proteins, Heat-Shock Proteins, Mice, Molecular Chaperones, Peptidylprolyl Isomerase, Protein Binding, Protein Structure, Tertiary, Recombinant Proteins, Tacrolimus Binding Proteins
FEBS Lett.
Date: Feb. 13, 2004
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