Mammalian formin-1 participates in adherens junctions and polymerization of linear actin cables.
During epithelial sheet formation, linear actin cables assemble at nascent adherens junctions. This process requires alpha-catenin and actin polymerization, although the underlying mechanism is poorly understood. Here, we show that formin-1 interacts with alpha-catenin, localizes to adherens junctions and nucleates unbranched actin filaments. Furthermore, disruption of the alpha-catenin-formin-1 interaction blocks ... assembly of radial actin cables and perturbs intercellular adhesion. A fusion protein of the beta-catenin-binding domain of alpha-catenin with the actin polymerization domains of formin-1 rescues formation of adherens junctions and associated actin cables in alpha-catenin-null keratinocytes. These findings provide new insight into how alpha-catenin orchestrates actin dynamics during intercellular junction formation.
Mesh Terms:
Actins, Adherens Junctions, Animals, Cell Adhesion, Cells, Cultured, Cytoskeletal Proteins, Epithelial Cells, Fetal Proteins, Keratinocytes, Mice, Microfilament Proteins, Microfilaments, Nuclear Proteins, Polymers, Protein Binding, Protein Structure, Tertiary, Recombinant Fusion Proteins, Trans-Activators, alpha Catenin, beta Catenin
Actins, Adherens Junctions, Animals, Cell Adhesion, Cells, Cultured, Cytoskeletal Proteins, Epithelial Cells, Fetal Proteins, Keratinocytes, Mice, Microfilament Proteins, Microfilaments, Nuclear Proteins, Polymers, Protein Binding, Protein Structure, Tertiary, Recombinant Fusion Proteins, Trans-Activators, alpha Catenin, beta Catenin
Nat. Cell Biol.
Date: Jan. 01, 2004
PubMed ID: 14647292
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