Identification and characterization of a nuclear interacting partner of anaplastic lymphoma kinase (NIPA).
Anaplastic large-cell lymphoma is a subtype of non-Hodgkin lymphomas characterized by the expression of CD30. More than half of these lymphomas carry a chromosomal translocation t(2;5) leading to expression of the oncogenic tyrosine kinase nucleophosmin-anaplastic lymphoma kinase (NPM-ALK). NPM-ALK is capable of transforming fibroblasts and lymphocytes in vitro and of ... causing lymphomas in mice. Previously, we and others demonstrated phospholipase C-gamma and phosphatidylinositol 3-kinase as crucial downstream signaling mediators of NPM-ALK-induced oncogenicity. In this study, we used an ALK fusion protein as bait in a yeast two-hybrid screen identifying NIPA (nuclear interacting partner of ALK) as a novel downstream target of NPM-ALK. NIPA encodes a 60-kDa protein that is expressed in a broad range of human tissues and contains a classical nuclear translocation signal in its C terminus, which directs its nuclear localization. NIPA interacts with NPM-ALK and other ALK fusions in a tyrosine kinase-dependent manner and is phosphorylated in NPM-ALK-expressing cells on tyrosine and serine residues with serine 354 as a major phosphorylation site. Overexpression of NIPA in Ba/F3 cells was able to protect from apoptosis induced by IL-3 withdrawal. Mutations of the nuclear translocation signal or the Ser-354 phosphorylation site impaired the antiapoptotic function of NIPA. In NPM-ALK-transformed Ba/F3 cells, apoptosis triggered by wortmannin treatment was enhanced by overexpression of putative dominant-negative NIPA mutants. These results implicate an antiapoptotic role for NIPA in NPM-ALK-mediated signaling events.
Mesh Terms:
Active Transport, Cell Nucleus, Adaptor Proteins, Signal Transducing, Amino Acid Sequence, Androstadienes, Animals, Antigens, CD30, Apoptosis, Blotting, Northern, COS Cells, Cell Cycle, Cell Line, Cell Nucleus, Cloning, Molecular, DNA, Complementary, Electrophoresis, Polyacrylamide Gel, Enzyme Inhibitors, Fibroblasts, Glutathione Transferase, Humans, Immunoblotting, In Situ Nick-End Labeling, Lymphoma, Large B-Cell, Diffuse, Mice, Microscopy, Fluorescence, Models, Biological, Molecular Sequence Data, Mutation, Nuclear Proteins, Phosphatidylinositol 3-Kinases, Phosphoamino Acids, Phospholipase C gamma, Phosphorylation, Plasmids, Protein Binding, Protein Structure, Tertiary, Protein-Tyrosine Kinases, RNA, Messenger, Recombinant Fusion Proteins, Sequence Homology, Amino Acid, Serine, Signal Transduction, Time Factors, Tissue Distribution, Transfection, Two-Hybrid System Techniques, Type C Phospholipases, Tyrosine
Active Transport, Cell Nucleus, Adaptor Proteins, Signal Transducing, Amino Acid Sequence, Androstadienes, Animals, Antigens, CD30, Apoptosis, Blotting, Northern, COS Cells, Cell Cycle, Cell Line, Cell Nucleus, Cloning, Molecular, DNA, Complementary, Electrophoresis, Polyacrylamide Gel, Enzyme Inhibitors, Fibroblasts, Glutathione Transferase, Humans, Immunoblotting, In Situ Nick-End Labeling, Lymphoma, Large B-Cell, Diffuse, Mice, Microscopy, Fluorescence, Models, Biological, Molecular Sequence Data, Mutation, Nuclear Proteins, Phosphatidylinositol 3-Kinases, Phosphoamino Acids, Phospholipase C gamma, Phosphorylation, Plasmids, Protein Binding, Protein Structure, Tertiary, Protein-Tyrosine Kinases, RNA, Messenger, Recombinant Fusion Proteins, Sequence Homology, Amino Acid, Serine, Signal Transduction, Time Factors, Tissue Distribution, Transfection, Two-Hybrid System Techniques, Type C Phospholipases, Tyrosine
J. Biol. Chem.
Date: Aug. 08, 2003
PubMed ID: 12748172
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