Identification of a cardiac isoform of the murine calcium channel alpha1C (Cav1.2-a) subunit and its preferential binding with the beta2 subunit.

We describe a cardiac muscle isoform of the voltage-dependent calcium channel alpha1 subunit, which corresponds to the rabbit ortholog of alpha1C-a (Cav1.2a). We also cloned smooth muscle isoforms alpha1C-b (Cav1.2b) and alpha1C-d (Cav1.2d). Differences among these three isoforms lie within the N-terminal region (exon 1A or 1B), the sixth transmembrane ...
segment of domain I (exon 8A or 8B), and the use of exon 10, which forms the intracellular loop between transmembrane domains I and II. Two-hybrid analysis revealed interactions among the three alpha1 isoforms and beta subunits. In vitro overlay and immunoprecipitation analyses revealed preferential binding between alpha1C-a and beta2, which is also expressed at a high level in the heart.
Mesh Terms:
Alternative Splicing, Amino Acid Sequence, Animals, Base Sequence, Calcium Channels, L-Type, Exons, Mice, Mice, Inbred C57BL, Molecular Sequence Data, Muscle, Smooth, Myocardium, Polymerase Chain Reaction, Protein Isoforms, Protein Structure, Tertiary, Protein Subunits, Sequence Deletion, Sequence Homology, Amino Acid
J. Mol. Cell. Cardiol.
Date: Jul. 01, 2006
Download Curated Data For This Publication
120338
Switch View:
  • Interactions 7