The archetype STYX/dead-phosphatase complexes with a spermatid mRNA-binding protein and is essential for normal sperm production.
Differentiation of spermatids into spermatozoa is regulated via phosphorylated RNA-binding proteins that modulate the expression of stage-specific mRNAs. We demonstrate that the phosphoserine, -threonine or -tyrosine, interaction protein, Styx, complexes with a testicular RNA-binding protein and is essential for normal spermiogenesis. Ablation of Styx expression in mouse disrupts round and ... elongating spermatid development, resulting in a >1,000-fold decrease in spermatozoa production. Moreover, Styx(-/-) males are infertile because of structural head abnormalities in residual epididymal sperm. Immunoprecipitation of Styx with Crhsp-24, a phosphorylated RNA-binding protein implicated in translational repression of histone mRNAs, provides a strategy for regulating posttranscriptional gene expression.
Mesh Terms:
Animals, Blotting, Northern, Blotting, Western, Carrier Proteins, DNA-Binding Proteins, Genetic Vectors, Humans, Male, Mice, Mice, Inbred C57BL, Models, Genetic, Phosphoproteins, Phosphorylation, Plasmids, Precipitin Tests, Protein Structure, Tertiary, RNA, RNA Processing, Post-Transcriptional, Spermatogenesis, Spermatozoa, Testis, Transcription Factors
Animals, Blotting, Northern, Blotting, Western, Carrier Proteins, DNA-Binding Proteins, Genetic Vectors, Humans, Male, Mice, Mice, Inbred C57BL, Models, Genetic, Phosphoproteins, Phosphorylation, Plasmids, Precipitin Tests, Protein Structure, Tertiary, RNA, RNA Processing, Post-Transcriptional, Spermatogenesis, Spermatozoa, Testis, Transcription Factors
Proc. Natl. Acad. Sci. U.S.A.
Date: Feb. 19, 2002
PubMed ID: 11842224
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