CatSperbeta, a novel transmembrane protein in the CatSper channel complex.
Four CatSper ion channel subunit genes (CatSpers 1-4) are required for sperm cell hyperactivation and male fertility. The four proteins assemble (presumably as a tetramer) to form a sperm-specific, alkalinization-activated Ca(2+)-selective channel. We set out to identify proteins associating with CatSper that might help explain its unique role in spermatozoa. ... Using a transgenic approach, a CatSper1 complex was purified from mouse testis that contained heat shock protein 70-2, a testis-specific chaperone, and CatSperbeta, a novel protein with two putative transmembrane-spanning domains. Like the CatSper ion channel subunits, CatSperbeta was restricted to testis and localized to the principal piece of the sperm tail. CatSperbeta protein is absent in CatSper1(-/-) sperm, suggesting that it is required for trafficking or formation of a stable channel complex. CatSperbeta is the first identified auxiliary protein to the CatSper channel.
Mesh Terms:
Amino Acid Sequence, Animals, Calcium Channels, Cell Line, Female, HSP70 Heat-Shock Proteins, Humans, Kidney, Male, Membrane Proteins, Mice, Mice, Inbred C57BL, Mice, Transgenic, Molecular Sequence Data, Multiprotein Complexes, Patch-Clamp Techniques, Protein Transport, Sperm Tail
Amino Acid Sequence, Animals, Calcium Channels, Cell Line, Female, HSP70 Heat-Shock Proteins, Humans, Kidney, Male, Membrane Proteins, Mice, Mice, Inbred C57BL, Mice, Transgenic, Molecular Sequence Data, Multiprotein Complexes, Patch-Clamp Techniques, Protein Transport, Sperm Tail
J. Biol. Chem.
Date: Jun. 29, 2007
PubMed ID: 17478420
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