The identification of a nonclassical cadherin expressed during B cell development and its interaction with surrogate light chain.
A 130-kDa glycoprotein (p130) has been found to be associated with surrogate light chain on pro- and pre-B I cells. Using peptide sequences obtained from purified p130 we have cloned its gene. The gene encodes a typical cadherin type 1 membrane protein with six extracellular cadherin domains (one pseudo domain) ... but lacking the catenin-binding site in its cytoplasmic part. Even without this catenin-binding site, p130 mediates Ca(2+)-dependent homotypic adhesion of cells. The interaction of p130 with surrogate light chain is confirmed by co-transfection and co-immunoprecipitation experiments. The expression of p130 is biphasic during the B cell development. Reverse transcriptase-polymerase chain reaction and flow cytometric analyses revealed that it is expressed on B220(+)c-Kit(+) pro-B and pre-B-I cells as well as on B220(+)CD25(-)IgM(+) immature and mature B cells but not on B220(+)CD25(+) pre-B-II cells. It is also expressed in fetal liver, at low levels in myeloid cells, and strongly in intestinal epithelial cells. In the spleen, p130-expressing cells are mainly localized in the marginal zone. We call this B lineage-, intestine-, liver- and leukocyte-expressed gene BILL-cadherin. The possible functions of BILL-cadherin in B cell development are discussed.
Mesh Terms:
Amino Acid Sequence, Animals, Antibodies, Monoclonal, Antigens, CD45, B-Lymphocytes, Base Sequence, Binding Sites, Blotting, Northern, Cadherins, Calcium, Cell Adhesion, Cell Line, Cloning, Molecular, DNA, Complementary, Drosophila, Female, Flow Cytometry, Gene Library, Immunoglobulin Light Chains, Immunoglobulin Light Chains, Surrogate, Immunoglobulin M, Immunoglobulin Variable Region, Intestines, Leukocytes, Liver, Membrane Glycoproteins, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Molecular Sequence Data, Precipitin Tests, Protein Structure, Tertiary, Protein Tyrosine Phosphatase, Non-Receptor Type 1, Proto-Oncogene Proteins c-kit, Rats, Receptors, Interleukin-2, Reverse Transcriptase Polymerase Chain Reaction, Time Factors, Tissue Distribution, Transfection
Amino Acid Sequence, Animals, Antibodies, Monoclonal, Antigens, CD45, B-Lymphocytes, Base Sequence, Binding Sites, Blotting, Northern, Cadherins, Calcium, Cell Adhesion, Cell Line, Cloning, Molecular, DNA, Complementary, Drosophila, Female, Flow Cytometry, Gene Library, Immunoglobulin Light Chains, Immunoglobulin Light Chains, Surrogate, Immunoglobulin M, Immunoglobulin Variable Region, Intestines, Leukocytes, Liver, Membrane Glycoproteins, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Molecular Sequence Data, Precipitin Tests, Protein Structure, Tertiary, Protein Tyrosine Phosphatase, Non-Receptor Type 1, Proto-Oncogene Proteins c-kit, Rats, Receptors, Interleukin-2, Reverse Transcriptase Polymerase Chain Reaction, Time Factors, Tissue Distribution, Transfection
J. Biol. Chem.
Date: Oct. 06, 2000
PubMed ID: 10906147
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