Amyloid precursor proteins anchor CPEB to membranes and promote polyadenylation-induced translation.
The cytoplasmic polyadenylation element (CPE) binding factor, CPEB, is a sequence-specific RNA binding protein that controls polyadenylation-induced translation in germ cells and at postsynaptic sites of neurons. A yeast two-hybrid screen with a mouse brain cDNA library identified the transmembrane amyloid precursor-like protein 1 (APLP1) as a CPEB-interacting factor. CPEB ... binds the small intracellular domain (ICD) of APLP1 and the related proteins APLP2 and APP. These proteins promote polyadenylation and translation by stimulating Aurora A catalyzed CPEB serine 174 phosphorylation. Surprisingly, CPEB, Maskin, CPSF, and several other factors involved in polyadenylation and translation and CPE-containing RNA are all detected on membranes by cell fractionation and immunoelectron microscopy. Moreover, most of the RNA that undergoes polyadenylation does so in membrane-containing fractions. These data demonstrate a link between cytoplasmic polyadenylation and membrane association and implicate APP family member proteins as anchors for localized mRNA polyadenylation and translation.
Mesh Terms:
Amyloid beta-Protein Precursor, Animals, Cell Membrane, Cytoplasm, Gene Library, Mice, Neurons, Phosphorylation, Polyadenylation, Protein Biosynthesis, Protein-Serine-Threonine Kinases, RNA, Messenger, RNA-Binding Proteins, Serine, Two-Hybrid System Techniques
Amyloid beta-Protein Precursor, Animals, Cell Membrane, Cytoplasm, Gene Library, Mice, Neurons, Phosphorylation, Polyadenylation, Protein Biosynthesis, Protein-Serine-Threonine Kinases, RNA, Messenger, RNA-Binding Proteins, Serine, Two-Hybrid System Techniques
Mol. Cell. Biol.
Date: Dec. 01, 2005
PubMed ID: 16314516
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