Molecular constituents of neuronal AMPA receptors.
Dynamic regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs) underlies aspects of synaptic plasticity. Although numerous AMPAR-interacting proteins have been identified, their quantitative and relative contributions to native AMPAR complexes remain unclear. Here, we quantitated protein interactions with neuronal AMPARs by immunoprecipitation from brain extracts. We found that stargazin-like transmembrane AMPAR regulatory ... proteins (TARPs) copurified with neuronal AMPARs, but we found negligible binding to GRIP, PICK1, NSF, or SAP-97. To facilitate purification of neuronal AMPAR complexes, we generated a transgenic mouse expressing an epitope-tagged GluR2 subunit of AMPARs. Taking advantage of this powerful new tool, we isolated two populations of GluR2 containing AMPARs: an immature complex with the endoplasmic reticulum chaperone immunoglobulin-binding protein and a mature complex containing GluR1, TARPs, and PSD-95. These studies establish TARPs as the auxiliary components of neuronal AMPARs.
Mesh Terms:
Animals, Brain, Calcium Channels, Cell Line, Heat-Shock Proteins, Humans, Intracellular Signaling Peptides and Proteins, Lymphokines, Membrane Proteins, Mice, Mice, Transgenic, Molecular Chaperones, Nerve Tissue Proteins, Neurons, Oocytes, Receptors, AMPA, Synaptic Membranes, Xenopus
Animals, Brain, Calcium Channels, Cell Line, Heat-Shock Proteins, Humans, Intracellular Signaling Peptides and Proteins, Lymphokines, Membrane Proteins, Mice, Mice, Transgenic, Molecular Chaperones, Nerve Tissue Proteins, Neurons, Oocytes, Receptors, AMPA, Synaptic Membranes, Xenopus
J. Cell Biol.
Date: May. 09, 2005
PubMed ID: 15883194
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