Mammalian Fat1 cadherin regulates actin dynamics and cell-cell contact.
Fat cadherins form a distinct subfamily of the cadherin gene superfamily, and are featured by their unusually large extracellular domain. In this work, we investigated the function of a mammalian Fat cadherin. Fat1 was localized at filopodial tips, lamellipodial edges, and cell-cell boundaries, overlapping with dynamic actin structures. RNA interference-mediated ... knockdown of Fat1 resulted in disorganization of cell junction-associated F-actin and other actin fibers/cables, disturbance of cell-cell contacts, and also inhibition of cell polarity formation at wound margins. Furthermore, we identified Ena/vasodilator-stimulated phosphoproteins as a potential downstream effector of Fat1. These results suggest that Fat1 regulates actin cytoskeletal organization at cell peripheries, thereby modulating cell contacts and polarity.
Mesh Terms:
Actins, Animals, Binding Sites, Cadherins, Cell Adhesion, Cell Adhesion Molecules, Cell Line, Cell Polarity, Fetus, Humans, Intercellular Junctions, Mice, Microfilaments, Protein Binding, Protein Structure, Tertiary, Pseudopodia, Wound Healing
Actins, Animals, Binding Sites, Cadherins, Cell Adhesion, Cell Adhesion Molecules, Cell Line, Cell Polarity, Fetus, Humans, Intercellular Junctions, Mice, Microfilaments, Protein Binding, Protein Structure, Tertiary, Pseudopodia, Wound Healing
J. Cell Biol.
Date: May. 24, 2004
PubMed ID: 15148305
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