ARF6-dependent interaction of the TWIK1 K+ channel with EFA6, a GDP/GTP exchange factor for ARF6.
TWIK1 belongs to a family of K(+) channels involved in neuronal excitability and cell volume regulation. Its tissue distribution suggests a role in epithelial potassium transport. Here we show that TWIK1 is expressed in a subapical compartment in renal proximal tubules and in polarized MDCK cells. In nonpolarized cells, this ... compartment corresponds to pericentriolar recycling endosomes. We identified EFA6, an exchange factor for the small G protein ADP-ribosylation factor 6 (ARF6), as a protein binding to TWIK1. EFA6 interacts with TWIK1 only when it is bound to ARF6. Because ARF6 modulates endocytosis at the apical surface of epithelial cells, the ARF6/EFA6/TWIK1 association is probably important for channel internalization and recycling.
Mesh Terms:
ADP-Ribosylation Factors, Animals, Endocytosis, Fluorescent Antibody Technique, Guanine Nucleotide Exchange Factors, Guanosine Diphosphate, Guanosine Triphosphate, Hela Cells, Humans, Kidney, Mice, Nerve Tissue Proteins, Peptide Elongation Factors, Potassium Channels, Transferrin
ADP-Ribosylation Factors, Animals, Endocytosis, Fluorescent Antibody Technique, Guanine Nucleotide Exchange Factors, Guanosine Diphosphate, Guanosine Triphosphate, Hela Cells, Humans, Kidney, Mice, Nerve Tissue Proteins, Peptide Elongation Factors, Potassium Channels, Transferrin
EMBO Rep.
Date: Dec. 01, 2004
PubMed ID: 15540117
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