Direct interaction between metastasis-associated protein 1 and endophilin 3.
The yeast two-hybrid system was used to search for partners of mouse metastasis-associated protein 1 (Mta1). Screening of a cDNA library prepared from mouse embryo yielded positive clones coding for endophilin 3. The site of interaction was suggested to be the SH-3-binding domain of Mta1 and SH-3 domain of endophilin ... 3. This interaction was confirmed by GST pull-down assay in vitro and immunoprecipitation in vivo. The Mta1 and endophilin 3 transcripts were highly expressed in testis and brain. But, Mta1 localized mainly in nucleus and to a lesser extent in cytoplasm while endophilin 3 localized mainly in cytoplasm. If Mta1 functions in cytoplasm, it might be involved in the regulation of endocytosis mediated by endophilin 3.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Animals, Brain, Brain Chemistry, Cell Cycle Proteins, Cell Nucleus, Cytoplasm, Female, Male, Mice, Mice, Inbred C57BL, Protein Interaction Mapping, RNA, Messenger, Testis, Tissue Distribution, Transcription Factors, Transcription, Genetic, Two-Hybrid System Techniques
Adaptor Proteins, Signal Transducing, Animals, Brain, Brain Chemistry, Cell Cycle Proteins, Cell Nucleus, Cytoplasm, Female, Male, Mice, Mice, Inbred C57BL, Protein Interaction Mapping, RNA, Messenger, Testis, Tissue Distribution, Transcription Factors, Transcription, Genetic, Two-Hybrid System Techniques
FEBS Lett.
Date: Jul. 04, 2005
PubMed ID: 15978591
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