Interaction between GADD34 and kinesin superfamily, KIF3A.
GADD34 is one of a subset of proteins induced after DNA damage or cell growth arrest. To examine the function of GADD34, we used the yeast two-hybrid system to clone the protein that interacts with the murine GADD34. One cDNA clone was the C-terminal part of KIF3A gene including the ... tail domain. The interaction between GADD34 and KIF3A was confirmed in the NIH3T3 cells by in vivo two-hybrid analysis. We could detect that GADD34 was induced with methyl methanesulfonate; however, the mRNA induction of KIF3A was not detected.
Mesh Terms:
3T3 Cells, Animals, Antigens, Differentiation, Cell Cycle Proteins, Cell Division, Cloning, Molecular, DNA Damage, DNA, Complementary, Kinesin, Mice, Protein Binding, Proteins, RNA, Messenger, Recombinant Proteins, Two-Hybrid System Techniques
3T3 Cells, Animals, Antigens, Differentiation, Cell Cycle Proteins, Cell Division, Cloning, Molecular, DNA Damage, DNA, Complementary, Kinesin, Mice, Protein Binding, Proteins, RNA, Messenger, Recombinant Proteins, Two-Hybrid System Techniques
Biochem. Biophys. Res. Commun.
Date: Jan. 19, 2000
PubMed ID: 10631107
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