PUMA couples the nuclear and cytoplasmic proapoptotic function of p53.
The Trp53 tumor suppressor gene product (p53) functions in the nucleus to regulate proapoptotic genes, whereas cytoplasmic p53 directly activates proapoptotic Bcl-2 proteins to permeabilize mitochondria and initiate apoptosis. Here, we demonstrate that a tripartite nexus between Bcl-xL, cytoplasmic p53, and PUMA coordinates these distinct p53 functions. After genotoxic stress, ... Bcl-xL sequestered cytoplasmic p53. Nuclear p53 caused expression of PUMA, which then displaced p53 from Bcl-xL, allowing p53 to induce mitochondrial permeabilization. Mutant Bcl-xL that bound p53, but not PUMA, rendered cells resistant to p53-induced apoptosis irrespective of PUMA expression. Thus, PUMA couples the nuclear and cytoplasmic proapoptotic functions of p53.
Mesh Terms:
Animals, Apoptosis, Apoptosis Regulatory Proteins, Cell Line, Tumor, Cell Nucleus, Cells, Cultured, Cytoplasm, DNA Damage, Gene Expression Regulation, Humans, Immunoprecipitation, Mice, Mice, Inbred C57BL, Mitochondria, Models, Biological, Permeability, Protein Binding, Protein Structure, Tertiary, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-bcl-2, Recombinant Proteins, Tumor Suppressor Protein p53, Tumor Suppressor Proteins, Ultraviolet Rays, bcl-2-Associated X Protein, bcl-X Protein
Animals, Apoptosis, Apoptosis Regulatory Proteins, Cell Line, Tumor, Cell Nucleus, Cells, Cultured, Cytoplasm, DNA Damage, Gene Expression Regulation, Humans, Immunoprecipitation, Mice, Mice, Inbred C57BL, Mitochondria, Models, Biological, Permeability, Protein Binding, Protein Structure, Tertiary, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-bcl-2, Recombinant Proteins, Tumor Suppressor Protein p53, Tumor Suppressor Proteins, Ultraviolet Rays, bcl-2-Associated X Protein, bcl-X Protein
Science
Date: Sep. 09, 2005
PubMed ID: 16151013
View in: Pubmed Google Scholar
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