Identification of novel retromer complexes in the mouse testis.
A family of vacuolar protein sorting (Vps) proteins, which are components of mammalian retromer complex, has been studied in the mouse. Vps26a is known as a retromer component that plays an important role in embryonic development: however, its cell-type expression and precise role remain to be elucidated. In this study, ... we identified a new isoform of Vps26a, called Vps26aT, which was expressed specifically in the mouse testis. Diverse expression patterns of Vps26 variants in mouse tissues were determined by Western blot and RT-PCR analyses, and the direct interaction of Vps26aT with Vps35 was also demonstrated by immunoprecipitation and pull-down assay using antibodies raised against each Vps component. Our results revealed that the retromer complex could be formed from different Vps26 isoforms in a tissue-specific manner, resulting in more than two types of the retromer complex, including the Vps26a-Vps29-Vps35, Vps26aT-Vps29-Vps35, and Vps26b-Vps29-Vps35 complexes in mouse tissues.
Mesh Terms:
Alternative Splicing, Amino Acid Sequence, Animals, Immunohistochemistry, Male, Mice, Molecular Sequence Data, Protein Isoforms, Testis, Tissue Distribution, Vesicular Transport Proteins
Alternative Splicing, Amino Acid Sequence, Animals, Immunohistochemistry, Male, Mice, Molecular Sequence Data, Protein Isoforms, Testis, Tissue Distribution, Vesicular Transport Proteins
Biochem. Biophys. Res. Commun.
Date: Oct. 10, 2008
PubMed ID: 18656452
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