Aurora B -TACC1 protein complex in cytokinesis.
Taxins are a family of centrosomal proteins important for the regulation of mitosis and microtubule dynamics. Cytokinesis, the last step of M phase, is essential for chromosomal integrity and cell division. It is highly regulated and involves a reorganization of microtubules and actin filaments. We show here that TACC1 localizes ... diffusely to the midzone spindle in anaphase and strongly to the midbody during cytokinesis, indicating a possible involvement of this protein in the exit of M phase. TACC1 also relocalizes to the nucleolus in interphase. We demonstrate that TACC1 and the mitotic kinase Aurora B belong to the same complex during cytokinesis. We further show that Aurora B knocked down by RNA-mediated interference prevents the formation of the midbody - and consequently affects TACC1 localization at this site - and leads to abnormal cell division and multinucleated cells.
Mesh Terms:
Anaphase, Cell Division, Cell Nucleus, Fetal Proteins, Hela Cells, Humans, Macromolecular Substances, Microtubule-Associated Proteins, Mitosis, Mitotic Spindle Apparatus, Nuclear Proteins, Protein Transport, Protein-Serine-Threonine Kinases, RNA Interference
Anaphase, Cell Division, Cell Nucleus, Fetal Proteins, Hela Cells, Humans, Macromolecular Substances, Microtubule-Associated Proteins, Mitosis, Mitotic Spindle Apparatus, Nuclear Proteins, Protein Transport, Protein-Serine-Threonine Kinases, RNA Interference
Oncogene
Date: Jun. 03, 2004
PubMed ID: 15064709
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