Werner syndrome protein participates in a complex with RAD51, RAD54, RAD54B and ATR in response to ICL-induced replication arrest.

Werner syndrome (WS) is a rare genetic disorder characterized by genomic instability caused by defects in the WRN gene encoding a member of the human RecQ helicase family. RecQ helicases are involved in several DNA metabolic pathways including homologous recombination (HR) processes during repair of stalled replication forks. Following introduction ...
of interstrand DNA crosslinks (ICL), WRN relocated from nucleoli to arrested replication forks in the nucleoplasm where it interacted with the HR protein RAD52. In this study, we use fluorescence resonance energy transfer (FRET) and immune-precipitation experiments to demonstrate that WRN participates in a multiprotein complex including RAD51, RAD54, RAD54B and ATR in cells where replication has been arrested by ICL. We verify the WRN-RAD51 and WRN-RAD54B direct interaction in vitro. Our data support a role for WRN also in the recombination step of ICL repair.
Mesh Terms:
Blotting, Western, Cell Cycle Proteins, Cell Nucleus, Cell Survival, Cross-Linking Reagents, DNA Helicases, DNA Replication, Enzyme-Linked Immunosorbent Assay, Exodeoxyribonucleases, Fluorescence Resonance Energy Transfer, Hela Cells, Humans, Immunoprecipitation, Luminescent Proteins, Microscopy, Confocal, Nuclear Proteins, Protein Binding, Protein Transport, Protein-Serine-Threonine Kinases, Rad51 Recombinase, RecQ Helicases, Recombinant Fusion Proteins
J. Cell. Sci.
Date: Dec. 15, 2006
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