A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology.
The Mediator is a multiprotein transcriptional coactivator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factors. In the work described here, we exploit multidimensional protein identification technology (MudPIT) to carry out a ... proteomic analysis of the subunit composition of the mammalian Mediator complex. By comparing MudPIT data sets obtained from six independent Mediator preparations immunoaffinity purified through their Nut2 (MED10), Med25 (MED9), Intersex (MED29), LCMR1 (MED19), AK007855 (MED28), or CRSP70 (MED26) subunits, we identify a set of consensus mammalian Mediator subunits. In addition, we identify as Mediator-associated proteins the CDK8-like cyclin-dependent kinase CDK11 and the TRAP240-like KIAA1025 protein (MED13L), which is mutated in patients with the congenital heart defect transposition of the great arteries (TGA).
Mesh Terms:
Adaptor Proteins, Signal Transducing, Carrier Proteins, Cyclin-Dependent Kinases, Drosophila Proteins, Hela Cells, Humans, Macromolecular Substances, Mediator Complex, Multiprotein Complexes, Nuclear Proteins, Protein Subunits, Proteomics, RNA Polymerase II, Saccharomyces cerevisiae Proteins, Trans-Activators, Transcription Factors
Adaptor Proteins, Signal Transducing, Carrier Proteins, Cyclin-Dependent Kinases, Drosophila Proteins, Hela Cells, Humans, Macromolecular Substances, Mediator Complex, Multiprotein Complexes, Nuclear Proteins, Protein Subunits, Proteomics, RNA Polymerase II, Saccharomyces cerevisiae Proteins, Trans-Activators, Transcription Factors
Mol. Cell
Date: Jun. 04, 2004
PubMed ID: 15175163
View in: Pubmed Google Scholar
Download Curated Data For This Publication
12145
Switch View:
- Interactions 255