FBP11 regulates nuclear localization of N-WASP and inhibits N-WASP-dependent microspike formation.
WASP family proteins are involved in cortical actin cytoskeleton reorganization. Neural Wiskott-Aldrich syndrome protein (N-WASP), a ubiquitously expressed WASP homologous protein, directly binds with Cdc42, activating Arp2/3 complex. In this study, we show that N-WASP-dependent microspike formation is inhibited by formin binding protein 11 (FBP11). Endogenous FBP11 localizes with nuclear-speckles, ... and co-localization of N-WASP and FBP11 was observed when they were co-expressed. Epidermal growth factor (EGF) induced actin-microspike formation in COS7 cells. However, transient expression of FBP11 suppressed N-WASP-dependent actin-microspike formation by trapping N-WASP in the nucleus. These results indicate that FBP11 regulates localization of N-WASP, thus negatively regulating the function of N-WASP in the cytoplasm.
Mesh Terms:
Actin-Related Protein 2, Actin-Related Protein 3, Actins, Amino Acid Sequence, Animals, COS Cells, Cell Nucleus, Cloning, Molecular, Cytoplasm, Cytoskeletal Proteins, DNA, Complementary, Escherichia coli, Expressed Sequence Tags, Glutathione Transferase, Homeodomain Proteins, Mass Spectrometry, Mice, Microscopy, Fluorescence, Molecular Sequence Data, Nerve Tissue Proteins, Plant Proteins, Protein Binding, Protein Structure, Tertiary, Recombinant Proteins, Saccharomyces cerevisiae, Sequence Homology, Amino Acid, Swine, Time Factors, Transcription Factors, Wiskott-Aldrich Syndrome Protein, Neuronal, cdc42 GTP-Binding Protein
Actin-Related Protein 2, Actin-Related Protein 3, Actins, Amino Acid Sequence, Animals, COS Cells, Cell Nucleus, Cloning, Molecular, Cytoplasm, Cytoskeletal Proteins, DNA, Complementary, Escherichia coli, Expressed Sequence Tags, Glutathione Transferase, Homeodomain Proteins, Mass Spectrometry, Mice, Microscopy, Fluorescence, Molecular Sequence Data, Nerve Tissue Proteins, Plant Proteins, Protein Binding, Protein Structure, Tertiary, Recombinant Proteins, Saccharomyces cerevisiae, Sequence Homology, Amino Acid, Swine, Time Factors, Transcription Factors, Wiskott-Aldrich Syndrome Protein, Neuronal, cdc42 GTP-Binding Protein
Biochem. Biophys. Res. Commun.
Date: Jan. 16, 2004
PubMed ID: 14697212
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