ATAC and Mediator coactivators form a stable complex and regulate a set of non-coding RNA genes.
The Ada-Two-A-containing (ATAC) histone acetyltransferase and Mediator coactivator complexes regulate independent and distinct steps during transcription initiation and elongation. Here, we report the identification of a new stable molecular assembly formed between the ATAC and Mediator complexes in mouse embryonic stem cells. Moreover, we identify leucine zipper motif-containing protein 1 ... as a subunit of this meta-coactivator complex (MECO). Finally, we demonstrate that the MECO regulates a subset of RNA polymerase II-transcribed non-coding RNA genes. Our findings establish that transcription coactivator complexes can form stable subcomplexes to facilitate their combined actions on specific target genes.
Mesh Terms:
Animals, Cells, Cultured, Chemokines, C, DNA-Binding Proteins, Fibroblasts, Gene Expression Regulation, Leucine Zippers, Mass Spectrometry, Mediator Complex, Mice, Mice, Knockout, Multiprotein Complexes, Peptides, Protein Subunits, RNA, Untranslated
Animals, Cells, Cultured, Chemokines, C, DNA-Binding Proteins, Fibroblasts, Gene Expression Regulation, Leucine Zippers, Mass Spectrometry, Mediator Complex, Mice, Mice, Knockout, Multiprotein Complexes, Peptides, Protein Subunits, RNA, Untranslated
EMBO Rep.
Date: Jul. 01, 2010
PubMed ID: 20508642
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