Association of human kinesin superfamily protein member 4 with BRCA2-associated factor 35.
A large portion of human kinesin superfamily protein member 4 (KIF4) is associated with the nuclear matrix during the interphase, while a small portion is found in the cytoplasm. During mitosis, it is associated with chromosomes throughout the entire process. In the present study, we identified a protein that interacts ... with KIF4 using a yeast two-hybrid system, co-immunoprecipitation and co-fractionation. This protein is BRCA2-associated factor 35 (BRAF35) containing a non-specific DNA binding high-mobility-group domain and a kinesin-like coiled-coil domain. It appeared that the interaction between the two proteins occurs through their respective alpha-helical coiled-coil domains. The co-fractionation experiment revealed that KIF4 and BRAF35 were present in a complex of approx. 540 kDa. The composition and biological significance of this complex should be studied further.
Mesh Terms:
Amino Acid Sequence, BRCA2 Protein, DNA-Binding Proteins, Gene Library, Genetic Vectors, Hela Cells, Humans, Kinesin, Leucine Zippers, Molecular Sequence Data, Neoplasm Proteins, Peptides, Protein Binding, Protein Interaction Mapping, Protein Structure, Tertiary, Saccharomyces cerevisiae, Tumor Cells, Cultured, Two-Hybrid System Techniques
Amino Acid Sequence, BRCA2 Protein, DNA-Binding Proteins, Gene Library, Genetic Vectors, Hela Cells, Humans, Kinesin, Leucine Zippers, Molecular Sequence Data, Neoplasm Proteins, Peptides, Protein Binding, Protein Interaction Mapping, Protein Structure, Tertiary, Saccharomyces cerevisiae, Tumor Cells, Cultured, Two-Hybrid System Techniques
Biochem. J.
Date: Sep. 01, 2003
PubMed ID: 12809554
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