Ca2(+)-dependent interaction of N-copine, a member of the two C2 domain protein family, with OS-9, the product of a gene frequently amplified in osteosarcoma.

N-copine is a novel two C2 domain protein that shows Ca2(+)-dependent phospholipid binding and membrane association. By using yeast two-hybrid assays, we identified OS-9 as a protein capable of interacting with N-copine. We further revealed that the second C2 domain of N-copine bound with the carboxy-terminal region of OS-9. Their ...
interaction in vivo was also confirmed by co-immunoprecipitation from 293E cells co-expressing transfected N-copine and OS-9. In vitro binding assays showed that this interaction was Ca2(+)-dependent. By Northern blot analysis, N-copine and OS-9 were co-expressed in the same regions of human brain. These results reveal that OS-9 is a potential target of N-copine.
Mesh Terms:
Binding Sites, Brain Chemistry, Calcium, Carrier Proteins, Cloning, Molecular, Gene Amplification, Humans, Neoplasm Proteins, Osteosarcoma, Protein Binding, Saccharomyces cerevisiae, Tissue Distribution
FEBS Lett.
Date: Jun. 18, 1999
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