Control of PKA stability and signalling by the RING ligase praja2.

Activation of G-protein-coupled receptors (GPCRs) mobilizes compartmentalized pulses of cyclic AMP. The main cellular effector of cAMP is protein kinase A (PKA), which is assembled as an inactive holoenzyme consisting of two regulatory (R) and two catalytic (PKAc) subunits. cAMP binding to R subunits dissociates the holoenzyme and releases the ...
catalytic moiety, which phosphorylates a wide array of cellular proteins. Reassociation of PKAc and R components terminates the signal. Here we report that the RING ligase praja2 controls the stability of mammalian R subunits. Praja2 forms a stable complex with, and is phosphorylated by, PKA. Rising cAMP levels promote praja2-mediated ubiquitylation and subsequent proteolysis of compartmentalized R subunits, leading to sustained substrate phosphorylation by the activated kinase. Praja2 is required for efficient nuclear cAMP signalling and for PKA-mediated long-term memory. Thus, praja2 regulates the total concentration of R subunits, tuning the strength and duration of PKA signal output in response to cAMP.
Mesh Terms:
A Kinase Anchor Proteins, Animals, Cell Line, Tumor, Cyclic AMP, Cyclic AMP Response Element-Binding Protein, Cyclic AMP-Dependent Protein Kinases, DNA-Binding Proteins, Enzyme Activation, Enzyme Stability, HEK293 Cells, Humans, Long-Term Potentiation, Mice, Neuroblastoma, Neurons, Protein Subunits, Proto-Oncogene Proteins c-fos, Rats, Recombinant Fusion Proteins, Signal Transduction, Two-Hybrid System Techniques, Ubiquitin-Protein Ligases
Nat. Cell Biol.
Date: Apr. 01, 2011
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