Cloning and sequencing of a novel human gene which encodes a putative hydroxylase.
Using a conventional two-hybrid technique with MAWD as bait protein, a novel full-length cDNA was isolated and sequenced from a human liver cDNA library. This cDNA consists of 2,575 base pairs and has a predicted open reading frame encoding 255 amino acids. Overall, it is similar to the catalytic enzyme ... PHZF, catalyzing the hydroxylation of phenazine-1-carboxylic acid to 2-hydroxyphenazine-1-carboxylic acid. Polymerase chain reaction-based mapping with both a monochromosomal hybrid panel and radiation hybrid cell panels placed the gene to human chromosome 10q21.1 near the marker D10S210.
Mesh Terms:
Base Sequence, Chromosomes, Human, Pair 10, Cloning, Molecular, DNA, Complementary, Humans, Liver, Mixed Function Oxygenases, Molecular Sequence Data, Neoplasm Proteins, Organ Specificity, Phenazines, Physical Chromosome Mapping, Proteins, Radiation Hybrid Mapping, Sequence Analysis, DNA, Sequence Homology, Two-Hybrid System Techniques
Base Sequence, Chromosomes, Human, Pair 10, Cloning, Molecular, DNA, Complementary, Humans, Liver, Mixed Function Oxygenases, Molecular Sequence Data, Neoplasm Proteins, Organ Specificity, Phenazines, Physical Chromosome Mapping, Proteins, Radiation Hybrid Mapping, Sequence Analysis, DNA, Sequence Homology, Two-Hybrid System Techniques
J. Hum. Genet.
Date: May. 17, 2001
PubMed ID: 11355021
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