Cloning and sequencing of a novel human gene that encodes a putative target protein of Nesh-SH3.
By using a conventional two-hybrid technique with an Src homology 3 (SH3) domain of Nesh as the bait protein, a novel full-length cDNA was isolated and sequenced from a human placenta cDNA library. This cDNA consists of 3023 bp and has a predicted open reading frame that encodes 486 amino ... acids. It possesses an SH3 binding motif, a nuclear targeting sequence, and no catalytic domain. Overall, it has no similarity to known molecules involved in a signaling cascade. Polymerase Chair reaction-based mapping with both a monochromosomal hybrid panel and radiation hybrid cell panels localized the gene on human chromosome 3q12 near the marker D3S1271.
Mesh Terms:
Amino Acid Sequence, Base Pairing, Base Sequence, Carrier Proteins, Chromosomes, Human, Pair 3, Cloning, Molecular, Exons, Humans, Molecular Sequence Data, Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Two-Hybrid System Techniques, src Homology Domains
Amino Acid Sequence, Base Pairing, Base Sequence, Carrier Proteins, Chromosomes, Human, Pair 3, Cloning, Molecular, Exons, Humans, Molecular Sequence Data, Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Two-Hybrid System Techniques, src Homology Domains
J. Hum. Genet.
Date: Aug. 15, 2001
PubMed ID: 11501947
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