Trihydrophobin 1 attenuates androgen signal transduction through promoting androgen receptor degradation.

The androgen-signaling pathway plays critical roles in normal prostate development, benign prostatic hyperplasia, established prostate cancer, and in prostate carcinogenesis. In this study, we report that trihydrophobin 1 (TH1) is a potent negative regulator to attenuate the androgen signal-transduction cascade through promoting androgen receptor (AR) degradation. TH1 interacts with AR ...
both in vitro and in vivo, decreases the stability of AR, and promotes AR ubiquitination in a ligand-independent manner. TH1 also associates with AR at the active androgen-responsive prostate-specific antigen (PSA) promoter in the nucleus of LNCaP cells. Decrease of endogenous AR protein by TH1 interferes with androgen-induced luciferase reporter expression and reduces endogenous PSA expression. Taken together, these results indicate that TH1 is a novel regulator to control the duration and magnitude of androgen signal transduction and might be directly involved in androgen-related developmental, physiological, and pathological processes.
Mesh Terms:
Androgens, Animals, COS Cells, Carrier Proteins, Cell Line, Tumor, Cell Nucleus, Cercopithecus aethiops, Down-Regulation, Enhancer Elements, Genetic, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Humans, Mice, Mice, Inbred C57BL, Promoter Regions, Genetic, Prostate-Specific Antigen, Protein Binding, Protein Processing, Post-Translational, Protein Stability, RNA, Messenger, Receptors, Androgen, Signal Transduction, Transcriptional Activation, Ubiquitination
J. Cell. Biochem.
Date: Apr. 01, 2010
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