Characterization of Solt, a novel SoxLZ/Sox6 binding protein expressed in adult mouse testis.
SoxLZ/Sox6, a member of the Sox protein family, contains a leucine zipper motif in addition to an HMG box, which is its DNA binding domain. Here we have identified a novel SoxLZ/Sox6 binding protein, termed Solt, which we obtained independently using both a far-Western blot and a yeast two-hybrid screen. ... Like SoxLZ/Sox6 mRNA, Solt mRNA was exclusively expressed in the testis in mouse. Solt contains an unusual leucine zipper, which bound to the leucine zipper region of SoxLZ/Sox6 in vitro. In transient transfection assays in CHO cells with SoxLZ/Sox6 containing the transactivational region of herpes simplex virus VP16, expression of a reporter gene that carries a cis binding region for Sox proteins was significantly enhanced by the co-expression of Solt and Ca(2+)/calmodulin-dependent protein kinase IV.
Mesh Terms:
Amino Acid Sequence, Animals, Base Sequence, Binding Sites, CHO Cells, Calcium-Calmodulin-Dependent Protein Kinase Type 4, Calcium-Calmodulin-Dependent Protein Kinases, Cricetinae, DNA-Binding Proteins, Enzyme Activation, Fish Proteins, Gene Expression Regulation, Genes, Reporter, Herpes Simplex Virus Protein Vmw65, High Mobility Group Proteins, Leucine Zippers, Male, Mice, Molecular Sequence Data, Mutation, Organ Specificity, Promoter Regions, Genetic, Protein Binding, RNA, Messenger, Recombinant Fusion Proteins, SOX Transcription Factors, SOXD Transcription Factors, Testis, Transcription Factors, Transfection, Two-Hybrid System Techniques
Amino Acid Sequence, Animals, Base Sequence, Binding Sites, CHO Cells, Calcium-Calmodulin-Dependent Protein Kinase Type 4, Calcium-Calmodulin-Dependent Protein Kinases, Cricetinae, DNA-Binding Proteins, Enzyme Activation, Fish Proteins, Gene Expression Regulation, Genes, Reporter, Herpes Simplex Virus Protein Vmw65, High Mobility Group Proteins, Leucine Zippers, Male, Mice, Molecular Sequence Data, Mutation, Organ Specificity, Promoter Regions, Genetic, Protein Binding, RNA, Messenger, Recombinant Fusion Proteins, SOX Transcription Factors, SOXD Transcription Factors, Testis, Transcription Factors, Transfection, Two-Hybrid System Techniques
FEBS Lett.
Date: Sep. 15, 2000
PubMed ID: 10996314
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