Cloning and characterization of a novel gene which encodes a protein interacting with the mitosis-associated kinase-like protein NTKL.

NTKL is an evolutionarily conserved kinase-like protein. The cell-cycle-dependent centrosomal localization of NTKL suggested that it was involved in centrosome-related cellular function. The mouse NTKL protein is highly homologous with human NTKL. A novel mouse protein was identified as an NTKL-binding protein (NTKL-BP1) by yeast two-hybrid screening, and the full-length ...
cDNA was amplified based on the result of a sequence data analysis cloning strategy. The full-length cDNA sequence of the NTKL-BP1 gene consists of 2,537 bp, which encode 368 amino acids. A database search revealed that homologues of NTKL-BP1 exist in different organisms, including Arabidopsis thaliana, Drosophila melanogaster, Plasmodium falciparum, Geobacter metallireducens, Anopheles gambiae and human. It suggests that NTKL-BP1 is an evolutionarily conserved protein. The expression of NTKL-BP1 was observed in multiple normal mouse tissues. The interaction of the two proteins was confirmed by co-immunoprecipitation. Moreover, immunofluorescent staining indicated that NTKL and NTKL-BP1 were all localized in the cytoplasm.
Mesh Terms:
Amino Acid Sequence, Animals, Base Sequence, Carrier Proteins, Cell Line, Tumor, Cloning, Molecular, Cytoplasm, Databases, Genetic, Fluorescent Antibody Technique, Direct, Mice, Mitosis, Molecular Sequence Data, Precipitin Tests, Protein Kinases, Protein Structure, Tertiary, Sequence Homology, Amino Acid, Tissue Distribution, Two-Hybrid System Techniques
J. Hum. Genet.
Date: Jun. 05, 2003
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