Human TAFII 105 is a cell type-specific TFIID subunit related to hTAFII130.
We previously characterized Drosophila and human TAF subunits that make up the core TFIID complex found in all cells. Here, we report that differentiated B cells contain a novel substoichiometric TAF of 105 kDa not found associated with TFIID isolated from other cell types. The cDNA encoding hTAFII105 reveals a ... highly conserved C-terminal domain shared by hTAFII130 and oTAFII110, while the N-terminal coactivator domain has diverged significantly. All cells tested express TAFII105 mRNA, but only B cells contain significant levels of protein associated with TFIID. Transient overexpression of hTAFII105 selectively squelches the transcription of some genes in B cells. These properties suggest that TAFII105 is a cell type-specific subunit of TFIID that may be responsible for mediating transcription by a subset of activators in B cells.
Mesh Terms:
Amino Acid Sequence, Animals, B-Lymphocytes, Cell Differentiation, Cell Line, Cloning, Molecular, DNA, Complementary, DNA-Binding Proteins, Drosophila, Drosophila Proteins, Gene Expression, Humans, Molecular Sequence Data, Promoter Regions, Genetic, RNA, Messenger, Recombinant Fusion Proteins, Sequence Analysis, DNA, Sequence Homology, Amino Acid, TATA-Binding Protein Associated Factors, Trans-Activators, Transcription Factor TFIID, Transcription Factors
Amino Acid Sequence, Animals, B-Lymphocytes, Cell Differentiation, Cell Line, Cloning, Molecular, DNA, Complementary, DNA-Binding Proteins, Drosophila, Drosophila Proteins, Gene Expression, Humans, Molecular Sequence Data, Promoter Regions, Genetic, RNA, Messenger, Recombinant Fusion Proteins, Sequence Analysis, DNA, Sequence Homology, Amino Acid, TATA-Binding Protein Associated Factors, Trans-Activators, Transcription Factor TFIID, Transcription Factors
Cell
Date: Oct. 04, 1996
PubMed ID: 8858156
View in: Pubmed Google Scholar
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