Human cyclin-dependent kinase-activating kinase exists in three distinct complexes.
Transcription factor IIH (TFIIH) is a multisubunit complex required for transcription and for DNA nucleotide excision repair. TFIIH possesses three enzymatic activities: (i) an ATP-dependent DNA helicase, (ii) a DNA-dependent ATPase, and (iii) a kinase with specificity for the carboxyl-terminal domain of RNA polymerase II. The kinase activity was recently ... identified as the cdk (cyclin-dependent kinase) activating kinase, CAK, composed of cdk7, cyclin H, and MAT-1. Here we report the isolation and characterization of three distinct CAK-containing complexes from HeLa nuclear extracts: CAK, a novel CAK-ERCC2 complex, and TFIIH. CAK-ERCC2 can efficiently associate with core-TFIIH to reconstitute holo-TFIIH transcription activity. We present evidence proposing a critical role for ERCC2 in mediating the association of CAK with core TFIIH subunits.
Mesh Terms:
Cyclin-Dependent Kinases, Enzyme Activation, Hela Cells, Humans, Isoenzymes, Transcription Factor TFIIH, Transcription Factors, Transcription Factors, TFII
Cyclin-Dependent Kinases, Enzyme Activation, Hela Cells, Humans, Isoenzymes, Transcription Factor TFIIH, Transcription Factors, Transcription Factors, TFII
Proc. Natl. Acad. Sci. U.S.A.
Date: Jun. 25, 1996
PubMed ID: 8692842
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