A novel docking site on Mediator is critical for activation by VP16 in mammalian cells.

ARC92/ACID1 was identified as a novel specific target of the herpes simplex transactivator VP16 using an affinity purification procedure. Characterization of the protein revealed tight interactions with human Mediator mediated through a von Willebrand type A domain. ARC92/ACID1 further contains a novel activator-interacting domain (ACID), which it shares with at ...
least one other human gene termed PTOV1/ACID2. The structure of ARC92/ACID1 is of ancient origin but is conserved in mammals and in selected higher eukaryotes. A subpopulation of Mediator is associated with ARC92/ACID1, which is specifically required for VP16 activation both in vitro and in mammalian cells, but is dispensable for other activators such as SP1. Despite many known targets of VP16, ARC92/ACID1 appears to impose a critical control on transcription activation by VP16 in mammalian cells.
Mesh Terms:
Animals, Cell Line, Cosmids, DNA, Complementary, Genetic Vectors, Hela Cells, Herpes Simplex Virus Protein Vmw65, Humans, Mice, Recombinant Proteins, Transcription, Genetic, Transfection
EMBO J.
Date: Dec. 15, 2003
Download Curated Data For This Publication
124788
Switch View:
  • Interactions 31