Regulation of Rap2A by the ubiquitin ligase Nedd4-1 controls neurite development.
Nedd4-1 is a "neuronal precursor cell expressed and developmentally downregulated protein" and among the most abundant E3 ubiquitin ligases in mammalian neurons. In analyses of conventional and conditional Nedd4-1-deficient mice, we found that Nedd4-1 plays a critical role in dendrite formation. Nedd4-1, the serine/threonine kinase TNIK, and Rap2A form a ... complex that controls Nedd4-1-mediated ubiquitination of Rap2A. Ubiquitination by Nedd4-1 inhibits Rap2A function, which reduces the activity of Rap2 effector kinases of the TNIK family and promotes dendrite growth. We conclude that a Nedd4-1/Rap2A/TNIK signaling pathway regulates neurite growth and arborization in mammalian neurons.
Mesh Terms:
Animals, Animals, Newborn, Cells, Cultured, Coculture Techniques, Endosomal Sorting Complexes Required for Transport, Gene Expression Regulation, Developmental, Green Fluorescent Proteins, Hippocampus, Humans, Membrane Potentials, Mice, Mice, Knockout, Microglia, Myelin Proteolipid Protein, Neurites, Neurons, Patch-Clamp Techniques, Protein Binding, Protein-Serine-Threonine Kinases, RNA, Small Interfering, Rats, Signal Transduction, Silver Staining, Subcellular Fractions, Transfection, Ubiquitin-Protein Ligases, rap GTP-Binding Proteins
Animals, Animals, Newborn, Cells, Cultured, Coculture Techniques, Endosomal Sorting Complexes Required for Transport, Gene Expression Regulation, Developmental, Green Fluorescent Proteins, Hippocampus, Humans, Membrane Potentials, Mice, Mice, Knockout, Microglia, Myelin Proteolipid Protein, Neurites, Neurons, Patch-Clamp Techniques, Protein Binding, Protein-Serine-Threonine Kinases, RNA, Small Interfering, Rats, Signal Transduction, Silver Staining, Subcellular Fractions, Transfection, Ubiquitin-Protein Ligases, rap GTP-Binding Proteins
Neuron
Date: Feb. 11, 2010
PubMed ID: 20159449
View in: Pubmed Google Scholar
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