The human PAF1 complex acts in chromatin transcription elongation both independently and cooperatively with SII/TFIIS.
Genetic and cell-based studies have implicated the PAF1 complex (PAF1C) in transcription-associated events, but there has been no evidence showing a direct role in facilitating transcription of a natural chromatin template. Here, we demonstrate an intrinsic ability of human PAF1C (hPAF1C) to facilitate activator (p53)- and histone acetyltransferase (p300)-dependent transcription ... elongation from a recombinant chromatin template in a biochemically defined RNA polymerase II transcription system. This represents a PAF1C function distinct from its established role in histone ubiquitylation and methylation. Importantly, we further demonstrate a strong synergy between hPAF1C and elongation factor SII/TFIIS and an underlying mechanism involving direct hPAF1C-SII interactions and cooperative binding to RNA polymerase II. Apart from a distinct PAF1C function, the present observations provide a molecular mechanism for the cooperative function of distinct transcription elongation factors in chromatin transcription.
Mesh Terms:
Chromatin, Humans, Nuclear Proteins, RNA Polymerase II, Transcription, Genetic, Transcriptional Elongation Factors, Tumor Suppressor Protein p53, p300-CBP Transcription Factors
Chromatin, Humans, Nuclear Proteins, RNA Polymerase II, Transcription, Genetic, Transcriptional Elongation Factors, Tumor Suppressor Protein p53, p300-CBP Transcription Factors
Cell
Date: Feb. 19, 2010
PubMed ID: 20178742
View in: Pubmed Google Scholar
Download Curated Data For This Publication
125055
Switch View:
- Interactions 28