Mechanisms of NKT cell anergy induction involve Cbl-b-promoted monoubiquitination of CARMA1.
Repeated injection of alpha-galactosylceramide, an agonistic ligand for natural killer T (NKT) cells, results in long-term unresponsiveness or anergy, which severely limits its clinical application. However, the molecular mechanisms leading to NKT anergy induction remain unclear. We show here that the decreased IFN-gamma production and failed tumor rejection observed in ... anergized NKT cells are rescued by Cbl-b deficiency. Cbl-b E3 ligase activity is critical for the anergy induction, as revealed by the similarity between Cbl-b(-/-) and its RING finger mutant NKT cells. Cbl-b binds and promotes monoubiquitination to CARMA1, a critical signaling molecule in NFkappaB activation. Ubiquitin conjugation to CARMA1 disrupts its complex formation with Bcl10 without affecting its protein stability. In addition, CARMA1(-/-) NKT cells are defective in IFN-gamma production. The study identifies an important signaling pathway linking Cbl-b-induced monoubiquitination to NFkappaB activation in NKT cell anergy induction, which may help design approaches for human cancer therapy.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Animals, CARD Signaling Adaptor Proteins, Clonal Anergy, Galactosylceramides, Humans, Melanoma, Experimental, Mice, Mice, Inbred C57BL, Mice, Knockout, Mice, Mutant Strains, NF-kappa B, Natural Killer T-Cells, Proto-Oncogene Proteins c-cbl, Signal Transduction, Ubiquitination
Adaptor Proteins, Signal Transducing, Animals, CARD Signaling Adaptor Proteins, Clonal Anergy, Galactosylceramides, Humans, Melanoma, Experimental, Mice, Mice, Inbred C57BL, Mice, Knockout, Mice, Mutant Strains, NF-kappa B, Natural Killer T-Cells, Proto-Oncogene Proteins c-cbl, Signal Transduction, Ubiquitination
Proc. Natl. Acad. Sci. U.S.A.
Date: Oct. 20, 2009
PubMed ID: 19815501
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