Purification of the fission yeast MCM(MCM-BP) complex and consequences of functional inactivation using temperature-sensitive mutants.

The MCM (mini-chromosome maintenance) complex is the core of the eukaryotic replicative helicase and comprises six proteins, Mcm2-Mcm7. In humans, a variant form of the complex has Mcm2 replaced by the MCM-BP protein. Recent results suggest that a similar complex exists in fission yeast with an essential role in DNA ...
replication and cell cycle progression. Here, we describe purification and subunit composition of the fission yeast MCM(Mcb1) complex. Using newly generated temperature-sensitive alleles, we show that loss of MCM(Mcb1) function leads to accumulation of DNA damage, checkpoint activation and cell cycle arrest, and provide evidence for a role for MCM(Mcb1) in meiosis. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: Mcb1physically interacts with MCM4 by pull down (View interaction) Mcb1physically interacts with MCM6 by pull down (View interaction) Mcb1physically interacts with MCM5 by pull down (View interaction) MCM4 and Mcb1physically interact by bimolecular fluorescence complementation (View interaction) Mcb1physically interacts with MCM4, MCM3, MCM5, MCM6 and MCM7 by tandem affinity purification (View interaction) Mcb1physically interacts with MCM7 by pull down (View interaction).
Unknown
Date: Oct. 25, 2011
Download Curated Data For This Publication
125733
Switch View:
  • Interactions 12