Ubiquitin Conjugating Enzyme Ubc9 is Involved in Protein Degradation of Redox Factor-1 (Ref-1).

Redox factor-1 (Ref-1) is a bifunctional protein playing an important role in both cellular redox regulation and DNA apurinic/apyrimidinic sites' repair. To find Ref-1interacting proteins (Rips), a yeast two-hybrid screening was performed by using Ref-1 redox domain as the 'bait', and five positive clones were obtained. One of them (Rip3) ...
was identified to be the ubiquitin-conjugating enzyme Ubc9. Simultaneous overexpression of Ubc9 in Hela cells dramatically inhibited the enhancement of AP-1 reporter gene by Ref-1. Western blot indicated that the protein level of Ref-1 dropped down as the result of simultaneous overexpression of Ubc9. These results suggest that Ubc9 is involved in the protein degradation of Ref-1, resulting in the downregulation of Ref-1 physiological function.
Mesh Terms:
Amino Acid Sequence, Animals, Blotting, Western, Chromosome Mapping, Chromosomes, Human, DNA-Binding Proteins, Female, Humans, Immunohistochemistry, In Situ Hybridization, Fluorescence, Ligases, Male, Mice, Molecular Sequence Data, Organ Specificity, Rad51 Recombinase, Recombinant Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Sequence Homology, Amino Acid, Spermatocytes, Synaptonemal Complex, Ubiquitin-Conjugating Enzymes, X Chromosome, Y Chromosome
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao
Date: Jul. 12, 2002
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