Proliferating cell nuclear antigen in the cytoplasm interacts with components of glycolysis and cancer.
Proliferating cell nuclear antigen (PCNA) is involved in a wide range of functions in the nucleus. However, a substantial amount of PCNA is also present in the cytoplasm, although their function is unknown. Here we show, through Far-Western blotting and mass spectrometry, that PCNA is associated with several cytoplasmic oncoproteins, ... including elongation factor, malate dehydrogenase, and peptidyl-prolyl isomerase. Surprisingly, PCNA is also associated with six glycolytic enzymes that are involved in the regulation of steps 4-9 in the glycolysis pathway.
Mesh Terms:
Blotting, Far-Western, Blotting, Western, Cell Line, Cell Line, Tumor, Cytoplasm, Electrophoresis, Gel, Two-Dimensional, Enzymes, Fluorescent Antibody Technique, Fructose-Bisphosphate Aldolase, Glyceraldehyde-3-Phosphate Dehydrogenases, Glycolysis, Green Fluorescent Proteins, Humans, Neoplasms, Oncogene Proteins, Phosphopyruvate Hydratase, Proliferating Cell Nuclear Antigen, Protein Binding, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Transfection
Blotting, Far-Western, Blotting, Western, Cell Line, Cell Line, Tumor, Cytoplasm, Electrophoresis, Gel, Two-Dimensional, Enzymes, Fluorescent Antibody Technique, Fructose-Bisphosphate Aldolase, Glyceraldehyde-3-Phosphate Dehydrogenases, Glycolysis, Green Fluorescent Proteins, Humans, Neoplasms, Oncogene Proteins, Phosphopyruvate Hydratase, Proliferating Cell Nuclear Antigen, Protein Binding, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Transfection
FEBS Lett.
Date: Oct. 22, 2010
PubMed ID: 20849852
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